Product Description |
Recombinant Human FGFR4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu22-Asp369. |
Product Background |
Fibroblast growth factor receptor 4 (FGF R4), also known as CD334, is a 110 kDa glycosylated transmembrane receptor tyrosine kinase.Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. |
Product Category |
Recombinant Protein |
Target |
FGFR4 |
Synonyms |
CD334; FGF R4; FGFR4; FGFR-4; MGC20292; JTK2; TKF |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu22-Asp369 |
Molecular Weight |
The protein has a predicted MW of 65.3 kDa. Due to glycosylation, the protein migrates to 80-95 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 13.05 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human FGFR4, hFc Tag captured on CM5 Chip via Protein A can bind Human Beta Klotho, His Tag with an affinity constant of 13.05 nM as determined in SPR assay (Biacore T200). |
|
|
Human FGFR4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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The purity of-Human FGFR4 is greater than 95% as determined by SEC-HPLC. |
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