Name: Human FGL1 Protein (FGL-HM211)
Brand: KactusBio
SKU: FGL-HM211-100UG
Price: 315.0 USD
Availability: InStock

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human LAG3, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human FGL1, hFc Tag with the EC50 of 0.28ug/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Asp64-Asn305

Catalog #FGL-HM211

Size

100UG

500UG

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Product Description	Recombinant Human FGL1 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Asp64-Asn305.
Product Background	Fibrinogen-like protein 1 (FGL-1) is a protein that is structurally related to fibrinogen. In humans, FLG-1 is encoded by the FGL1 gene.Fibrinogen-like protein 1 is a member of the fibrinogen family of proteins, which also includes fibrinogen, fibrinogen-like protein 2, and clotting factors V, VIII, and XIII.Fibrinogen-like Protein 1 is a major immune inhibitory ligand of LAG-3.
Product Category	Recombinant Protein / Antibody discovery

Protein	FGL1
Synonyms	FGL1; FGL-1; Hepassocin; HP-041; HFREP-1; LFIRE-1; HFREP1; Lag3 ligand
Accession	Q08830
Species	Human
Biotinylated	no
Amino Acid Range	Asp64-Asn305
Molecular Weight	The protein has a predicted MW of 54.8 kDa. Due to glycosylation, the protein migrates to 60-66 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human LAG3, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human FGL1, hFc Tag with the EC50 of 0.28ug/ml determined by ELISA. See testing image for detail.
Product Tag	N-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 20mM PB, 250mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human LAG3, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human FGL1, hFc Tag with the EC50 of 0.28ug/ml determined by ELISA.
	Human FGL1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human LTBR is greater than 90% as determined by SEC-HPLC.