Human FGL1 protein (FGL-HM411)

  • Express System: Expi293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human FGL1, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human LAG3, hFc Tag with the EC50 of 0.8μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp64-Asn305

Catalog: FGL-HM411

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Product Description Recombinant Human FGL1 protein is expressed from Expi293 with His tag and Avi tag at the N-terminal.; It contains Asp64-Asn305.
Product Category Recombinant Protein
Target FGL1
Synonyms FGL1; FGL-1; Hepassocin; HP-041; HFREP-1; LFIRE-1; HFREP1; Lag3 ligand
Gene/Protein ID Q08830
Species Human
Exact Sequence Asp64-Asn305
Molecular Weight The protein has a predicted MW of 31.9 kDa same as Tris-Bis PAGE result.
Activity Immobilized Human FGL1, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human LAG3, hFc Tag with the EC50 of 0.8μg/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human FGL1, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human LAG3, hFc Tag with the EC50 of 0.8μg/ml determined by ELISA.
Immobilized Human FGL1, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human LAG3, hFc Tag with the EC50 of 0.8μg/ml/0.8μg/ml/0.7μg/ml determined by ELISA.
The purity of Human FGL1 is greater than 95% as determined by SEC-HPLC.
Human FGL1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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