Human FGL1 Protein (FGL-HM411)

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human FGL1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human LAG3, hFc Tag with the EC50 of 75.1ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Asp64-Asn305

Catalog: FGL-HM411

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Product Description	Recombinant Human FGL1 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Asp64-Asn305.
Product Background	Fibrinogen-like protein 1 (FGL-1) is a protein that is structurally related to fibrinogen. In humans, FLG-1 is encoded by the FGL1 gene.Fibrinogen-like protein 1 is a member of the fibrinogen family of proteins, which also includes fibrinogen, fibrinogen-like protein 2, and clotting factors V, VIII, and XIII.Fibrinogen-like Protein 1 is a major immune.
Product Category	Recombinant Protein

Target	FGL1
Synonyms	FGL1; FGL-1; Hepassocin; HP-041; HFREP-1; LFIRE-1; HFREP1; Lag3 ligand
Gene/Protein ID	Q08830
Species	Human
Exact Sequence	Asp64-Asn305
Molecular Weight	The protein has a predicted MW of 31.9 kDa same as Tris-Bis PAGE result.
Activity	Immobilized Human FGL1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human LAG3, hFc Tag with the EC50 of 75.1ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human FGL1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human LAG3, hFc Tag with the EC50 of 75.1ng/ml determined by ELISA.
	The purity of Human FGL1 is greater than 95% as determined by SEC-HPLC.
	Human FGL1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.