Human FGL2 Protein (FGL-HM612)

  • Express System: HEK293
  • Product Tag: N-His-Avi,N-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human FGL2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 14ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Val205-Pro439

Catalog: FGL-HM612

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Product Description Recombinant Human FGL2 Protein is expressed from HEK293 with His-Avi tag and Flag tag at the N-Terminus.It contains Val205-Pro439.

Product Background

Fibrinogen-like protein 2 (FGL2) is a member of the fibrinogen-like protein family and possesses important regulatory functions in both innate and adaptive immune responses. FGL2 is overexpressed in glioma, and its expression level is negatively associated with the prognosis of glioma patients.

Product Category

Recombinant Protein

Target

FGL2

Gene/Protein ID

Q14314

Species

Human

Exact Sequence

Val205-Pro439
Molecular Weight The protein has a predicted MW of 31.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human FGL2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 14ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-His-Avi,N-Flag

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human FGL2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 14ng/ml determined by ELISA.
The purity of Human FGL2 is greater than 95% as determined by SEC-HPLC.
Human FGL2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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