Product Description |
Recombinant Human Fibronectin (32-602) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln32-Leu602. |
Product Background |
Fibronectin is a high molecular glycoprotein present in the blood, connective tissue and at cell surface. It is synthesized by many types of differentiated cells and is believed to be involved in the attachment of cells to the surrounding extracellular matrix. Fibronectin has affinity to the other main components of extracellular matrix, collagen and glycosaminoglycans. |
Product Category |
Recombinant Protein |
Target |
Fibronectin (32-602) |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln32-Leu602 |
Molecular Weight |
The protein has a predicted MW of 65.47 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human Fibronectin (32-602) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human Fibronectin (32-602) is greater than 95% as determined by SEC-HPLC. |
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