Human ficolin 1/FCN1 Protein (FCN-HM101)
Catalog: FCN-HM101
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| Product Description | Recombinant Human ficolin 1/FCN1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ala30-Ala326. |
Product Background |
The ficolins are members of a recently discovered family of host innate opsonins that can activate the lectin pathway of complement. The ficolins bind many ligands, although they are typically described as binding acetylated sugars. Ficolin-1 is the least-described of the Ficolins and is expressed by monocytes, granulocytes, and in the lungs. Ficolin-1 is found circulating at low concentrations in serum but is regarded primarily as a secretory molecule that exerts its function locally in inflamed tissues. |
Product Category |
Recombinant Protein |
Target |
ficolin 1/FCN1 |
Gene/Protein ID |
NP_001994.2 |
Species |
Human |
Exact Sequence |
Ala30-Ala326 |
| Molecular Weight | The protein has a predicted MW of 33.11 kDa. Due to glycosylation, the protein migrates to 34-38 kDa based on Tris-Bis PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22 um filtered solution in PBS, 350mM NaCl, 200mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
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Human ficolin 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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