Product Description |
Recombinant Human FLRT1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ile21-Pro524. |
Product Background |
Fibronectin leucine rich transmembrane (FLRT) proteins have dual properties as regulators of cell adhesion and potentiators of fibroblast growth factor (FGF) mediated signalling. FLRT1 is a target for tyrosine phosphorylation mediated by FGFR1 and implicate a non-receptor Src family kinase (SFK). |
Product Category |
Recombinant Protein |
Target |
FLRT1 |
Synonyms |
SPG68; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ile21-Pro524 |
Molecular Weight |
The protein has a predicted MW of 56.58 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human FLRT1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FLRT1 Antibody, mFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human FLRT1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FLRT1 Antibody, mFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test). |
|
|
Human FLRT1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human FLRT1 is greater than 95% as determined by SEC-HPLC. |
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