Human FLRT1 Protein (FRT-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human FLRT1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FLRT1 Antibody, mFc Tag with the EC50 of 3.5ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ile21-Pro524

Catalog: FRT-HM101

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Product Description	Recombinant Human FLRT1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ile21-Pro524.
Product Background	Fibronectin leucine rich transmembrane (FLRT) proteins have dual properties as regulators of cell adhesion and potentiators of fibroblast growth factor (FGF) mediated signalling. FLRT1 is a target for tyrosine phosphorylation mediated by FGFR1 and implicate a non-receptor Src family kinase (SFK).
Product Category	Recombinant Protein

Target	FLRT1
Gene/Protein ID	Q9NZU1
Species	Human
Exact Sequence	Ile21-Pro524
Molecular Weight	The protein has a predicted MW of 56.58 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human FLRT1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FLRT1 Antibody, mFc Tag with the EC50 of 3.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human FLRT1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FLRT1 Antibody, mFc Tag with the EC50 of 3.5ng/ml determined by ELISA.
	The purity of Human FLRT1 is greater than 95% as determined by SEC-HPLC.
	Human FLRT1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.