Human FLRT1 Protein (FRT-HM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human FLRT1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FLRT1 Antibody, mFc Tag with the EC50 of 3.5ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ile21-Pro524

Catalog: FRT-HM101

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Product Description Recombinant Human FLRT1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ile21-Pro524.

Product Background

Fibronectin leucine rich transmembrane (FLRT) proteins have dual properties as regulators of cell adhesion and potentiators of fibroblast growth factor (FGF) mediated signalling. FLRT1 is a target for tyrosine phosphorylation mediated by FGFR1 and implicate a non-receptor Src family kinase (SFK).

Product Category

Recombinant Protein

Target

FLRT1

Gene/Protein ID

Q9NZU1

Species

Human

Exact Sequence

Ile21-Pro524
Molecular Weight The protein has a predicted MW of 56.58 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human FLRT1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FLRT1 Antibody, mFc Tag with the EC50 of 3.5ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human FLRT1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FLRT1 Antibody, mFc Tag with the EC50 of 3.5ng/ml determined by ELISA.
The purity of Human FLRT1 is greater than 95% as determined by SEC-HPLC.
Human FLRT1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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