Human FLT3/Flk-2 Protein (FLT-HM230)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human FLT3 Ligand, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.18 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Asn27-Asn541

Catalog: FLT-HM230

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Product Description	Recombinant Human FLT3/Flk-2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asn27-Asn541.
Product Background	The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1.
Product Category	Recombinant Protein

Target	FLT3/Flk-2
Synonyms	CD135; FLK2; STK-1; FLT-3
Accession	P36888-1
Species	Human
Amino Acid Range	Asn27-Asn541
Molecular Weight	The protein has a predicted MW of 84.9 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human FLT3 Ligand, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.18 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Mouse FLT3 Ligand, His Tag captured on CM5 Chip via anti-his antibody can bind Human FLT3, hFc Tag with an affinity constant of 0.18 nM as determined in SPR assay (Biacore T200).
	Immobilized Human FLT3 Ligand, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA (QC Test).
	Human FLT3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.