ELISA dose-response curve showing binding of human FLT3 hFc-tagged receptor to His-tagged FLT3 ligand, measured by absorbance versus concentration.
Human FLT3 Ligand Protein (FLT-HE03L)
Human FLT3 Ligand Protein (FLT-HE03L)
Human FLT3 Ligand Protein (FLT-HE03L)
  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human FLT3 Ligand, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 21.7ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Thr27-Ala181
Catalog #FLT-HE03L
Regular price $297.00
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Human FLT3 Ligand Protein (FLT-HE03L) Summary

Product Description

Recombinant Human FLT3 Ligand Protein is expressed from E.coli without tag.It contains Thr27-Ala181.

Product Background

Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human FLT3 Ligand Protein (FLT-HE03L) Specifications/Details

Protein
FLT3 Ligand
Synonyms
FL; Flt3 ligand; Flt3L; FLT3LG; SL cytokine; Flt-3 Ligand; Flt-3L
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Thr27-Ala181
Molecular Weight
The protein has a predicted MW of 17.61 kDa same as Bis-Tris PAGE result.
Activity
Immobilized Human FLT3 Ligand, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 21.7ng/ml determined by ELISA. See testing image for detail.
Product Tag
No Tag
Expression System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human FLT3 Ligand, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 21.7ng/ml determined by ELISA. Immobilized Human FLT3 Ligand, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 21.7ng/ml determined by ELISA.
Human FLT3 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human FLT3 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The-ED50-was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is < 2.0 ng/ml. (QC Test) The-ED50-was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is < 2.0 ng/ml. (QC Test)
The purity of Human FLT3 Ligand is greater than 95% as determined by SEC-HPLC. The purity of Human FLT3 Ligand is greater than 95% as determined by SEC-HPLC.
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