Human FLT3 Ligand Protein (FLT-HE03L)

  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human FLT3 Ligand, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 46.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Thr27-Ala181

Catalog: FLT-HE03L

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Product Description Recombinant Human FLT3 Ligand Protein is expressed from E.coli without tag.It contains Thr27-Ala181.

Product Background

Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.

Product Category

Recombinant Protein

Target

FLT3 Ligand

Gene/Protein ID

P49771-1

Species

Human

Exact Sequence

Thr27-Ala181
Molecular Weight The protein has a predicted MW of 17.61 kDa same as Tris-Bis PAGE result.

Activity

Immobilized Human FLT3 Ligand, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 46.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

No Tag

Express System

E.coli
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human FLT3 Ligand, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 46.0ng/ml determined by ELISA.
The-ED50-was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is < 1.0 ng/ml. (QC Test)
The purity of Human FLT3 Ligand is greater than 95% as determined by SEC-HPLC.
Human FLT3 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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