Human FLT3 Ligand Protein (FLT-HM13L)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human FLT3 Ligand, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 30ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Exact Sequence: Thr27-Pro185

Catalog: FLT-HM13L

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Product Description Recombinant Human FLT3 Ligand Protein is expressed from Expi293 with His tag at the C-terminal.It contains Thr27-Pro185.
Product Category Recombinant Protein
Target Flt3 Ligand
Synonyms FL; Flt3 ligand; Flt3L; FLT3LG; SL cytokine
Gene/Protein ID P49771-1
Species Human
Exact Sequence Thr27-Pro185
Molecular Weight The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human FLT3 Ligand, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 30ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human FLT3 Ligand, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 30ng/ml determined by ELISA (QC Test).
The ED50 was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is ≤ 1.0 ng/ml.
The purity of Human FLT3 Ligand is greater than 95% as determined by SEC-HPLC.
Human FLT3 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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