Human FLT3 Ligand Protein (FLT-HM13L)
Human FLT3 Ligand Protein (FLT-HM13L)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human FLT3 Ligand, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Thr27-Pro185
Catalog #FLT-HM13L
Regular price $330.00
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Product Description

Recombinant Human FLT3 Ligand Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr27-Pro185.

Product Background

Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.

Product Category

Recombinant Protein / Antibody discovery

Protein

FLT3 Ligand

Synonyms

FL; Flt3 ligand; Flt3L; FLT3LG; SL cytokine

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Thr27-Pro185

Molecular Weight

The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human FLT3 Ligand, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human FLT3 Ligand, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA (QC Test). Immobilized Human FLT3 Ligand, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA (QC Test).
Human FLT3 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human FLT3 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The ED50 was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is < 2.0 ng/ml. The ED50 was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is < 2.0 ng/ml.
The purity of Human FLT3 Ligand is greater than 95% as determined by SEC-HPLC. The purity of Human FLT3 Ligand is greater than 95% as determined by SEC-HPLC.

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