Product Description |
Recombinant Human FLT3 Ligand Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr27-Pro185. |
Product Background |
Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. |
Product Category |
Recombinant Protein |
Target |
Flt3 Ligand |
Synonyms |
FL; Flt3 ligand; Flt3L; FLT3LG; SL cytokine |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Thr27-Pro185 |
Molecular Weight |
The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human FLT3 Ligand, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human FLT3 Ligand, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA (QC Test). |
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Human FLT3 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The ED50 was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is < 2.0 ng/ml. |
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The purity of Human FLT3 Ligand is greater than 95% as determined by SEC-HPLC. |
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