• Express System: HEK293
  • Purity: > 95% as determined by HPLC
  • Activity: Immobilized Human Frizzled-4 VLP at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Frizzled-4 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA.
  • Amino Acid Range: Met1-Val537
Catalog #FZD-HM04V
Price
Regular price $1,23500
Regular price Sale price $1,23500
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Size
Avantor VWR Fisher Scientific

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Human Frizzled-4/FZD4 VLP (FZD-HM04V) Summary

Product Description

 Recombinant Human Frizzled-4/FZD4 VLP is expressed from HEK293.It contains Met1-Val537 (It may have cross reaction with anti-His antibody).

Product Background

 Frizzled4 (FZD4) is a receptor for Norrin and WNT7A/B ligands, is expressed in endothelial cells (ECs), is required for blood-CNS-barrier function as well as CNS angiogenesis, and transduces β-catenin-dependent signaling. FZD4 associates with additional receptor complex components: Tetraspanin12 (TSPAN12) is a Norrin co-receptor that amplifies signaling in a ligand specific manner.

Human Frizzled-4/FZD4 VLP (FZD-HM04V) Specifications/Details

Protein
 Frizzled-4/FZD4
Synonyms
 Frizzled-4; Fz-4; hFz4; FzE4; CD344; FZD4; FZD4S; FEVR; GPCR; Fz4
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Met1-Val537
Molecular Weight
 The target protein has a predicted MW of 59.37 kDa.
Activity
 Immobilized Human Frizzled-4 VLP at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Frizzled-4 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA.
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22um filtered solution in PBS, 300mM L-arginine (pH 7.4). Notice: If you need it for immunization, water-soluble adjuvant is recommended.
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human Frizzled-4 VLP at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Frizzled-4 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA. Immobilized Human Frizzled-4 VLP at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Frizzled-4 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA.
The purity of Human Frizzled-4 VLP is greater than 95% as determined by SEC-HPLC. The purity of Human Frizzled-4 VLP is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...