Human FSTL3 Protein (FTS-HM1L3)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human FSTL3, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FSTL3 Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Exact Sequence: Met27-Val263

Catalog: FTS-HM1L3

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Product Description	Recombinant Human FSTL3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met27-Val263.
Product Background	Follistatin-like 3 (FSTL3) is a novel cytokine that regulates insulin sensitivity and counteracts activin/myostatin signalling. In the present study, regulation of FSTL3 in renal dysfunction was investigated in both human chronic kidney disease (CKD) and acute kidney dysfunction (AKD). Furthermore, mFSTL3 expression was analysed in insulin-sensitive tissues in a mouse model of CKD.
Product Category	Recombinant Protein

Target	FSTL3
Synonyms	FLRG; FLRGFSRP; Follistatin-like 3; FSTL3
Gene/Protein ID	O95633-1
Species	Human
Exact Sequence	Met27-Val263
Molecular Weight	The protein has a predicted MW of 26.1 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human FSTL3, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FSTL3 Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human FSTL3, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FSTL3 Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA (QC Test).
	Immobilized Human Activin A, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human FSTL3, His Tag with the EC50 of 7.6ng/ml determined by ELISA.
	Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50-for this effect is typically 5-25 ng/mL in the presence of 7.5 ng/mL rhActivin A.
	Human FSTL3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.