Product Description |
Recombinant Human FSTL3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met27-Val263. |
Product Background |
Follistatin-like 3 (FSTL3) is a novel cytokine that regulates insulin sensitivity and counteracts activin/myostatin signalling. In the present study, regulation of FSTL3 in renal dysfunction was investigated in both human chronic kidney disease (CKD) and acute kidney dysfunction (AKD). Furthermore, mFSTL3 expression was analysed in insulin-sensitive tissues in a mouse model of CKD. |
Product Category |
Recombinant Protein |
Target |
FSTL3 |
Synonyms |
FLRG; FLRGFSRP; Follistatin-like 3; FSTL3 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met27-Val263 |
Molecular Weight |
The protein has a predicted MW of 26.1 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human FSTL3, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FSTL3 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Immobilized Human FSTL3, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FSTL3 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA (QC Test). |
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Immobilized Human Activin A, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human FSTL3, His Tag with the EC50 of 7.6ng/ml determined by ELISA. |
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Human FSTL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50-for this effect is typically 5-25 ng/mL in the presence of 7.5 ng/mL rhActivin A. |
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