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Human Coagulation factor XI Protein (pro form) (FXI-HM101) Summary
Product Description |
Recombinant Human Coagulation factor XI Protein is expressed from HEK293 with His tag at the C-Terminus. The protein needs to be activated by Thermolysin for an activated form.It contains Glu19-Val625. |
Product Background |
Coagulation factor (F)XI has been described as a component of the early phase of the contact pathway of blood coagulation, acting downstream of factor XII.In contrast to its mild hemostatic activities, FXI is known to play a significant role in thrombosis, as it is a demonstrated independent risk factor for deep vein thrombosis, ischemic stroke, and myocardial infarction. A better understanding of the varied and complex role of FXI in both thrombosis and hemostasis will help to allow better prediction of bleeding risk in FXI-deficient patients and also informing the development of targeted agents to inhibit the thrombotic activities of FXI while preserving hemostasis. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human Coagulation factor XI Protein (pro form) (FXI-HM101) Specifications/Details
Protein |
Coagulation factor XI |
Synonyms |
Coagulation factor XI; FXI; PTA; F11; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu19-Val625 |
Molecular Weight |
The protein has a predicted MW of 69.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Coagulation factor XI, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Factor XI Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate, t-butyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin (Boc-IEGR-AMC). The specific activity is >100 pmoles/min/ug. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 25mM HEPES, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human Coagulation factor XI, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Factor XI Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. |
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Human Coagulation factor XI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Coagulation factor XI is greater than 95% as determined by SEC-HPLC. |
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