Human G-CSF Protein (CSF-HM201)

Immobilized Human G-CSF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA (QC Test).

Human G-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human G-CSF is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human G-CSF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA (QC Test).
Amino Acid Range: Thr31-Pro204

Catalog #CSF-HM201

Price

$297⁰⁰

Size

Order by 4pm ET for delivery on:

Human G-CSF Protein (CSF-HM201) Summary

Product Description

Recombinant Human G-CSF Protein is expressed from HEK293 with hFc (IgG1) tag at the N-terminus.It contains Thr31-Pro204.

Product Background

Granulocyte colony-stimulating factor (G-CSF) is an hemopoietic growth factor produced by fibroblasts, monocytes and endothelial cells. G-CSF is expressed by numerous cell types including neuronal, immune and endothelial cells. G-CSF is released in autocrine manner and binds to its receptor G-CSF-R which further activates numerous signaling transduction pathways including PI3K/AKT, JAK/STAT and MAP kinase, and thereby promote neuronal survival, proliferation, differentiation, mobilization of hematopoietic stem and progenitor cells.

Human G-CSF Protein (CSF-HM201) Specifications/Details

Protein	G-CSF
Synonyms	CSF3; GCSF; C17orf33; CSF3OS;;Colony stimulating factor-3; Colony stimulating factor 3
Accession	NP_757373.1
Species	Human
Biotinylated	no
Amino Acid Range	Thr31-Pro204
Molecular Weight	The protein has a predicted MW of 44.20 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human G-CSF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA (QC Test).
Product Tag	N-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human G-CSF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA (QC Test).
	Human G-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human G-CSF is greater than 95% as determined by SEC-HPLC.