Human G-CSF Protein (CSF-HM201)
  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human G-CSF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Thr31-Pro204
Catalog #CSF-HM201
Regular price $270.00
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Product Description

Recombinant Human G-CSF Protein is expressed from HEK293 with hFc tag at the N-terminus.It contains Thr31-Pro204.

Product Background

Granulocyte colony-stimulating factor (G-CSF) is an hemopoietic growth factor produced by fibroblasts, monocytes and endothelial cells. G-CSF is expressed by numerous cell types including neuronal, immune and endothelial cells. G-CSF is released in autocrine manner and binds to its receptor G-CSF-R which further activates numerous signaling transduction pathways including PI3K/AKT, JAK/STAT and MAP kinase, and thereby promote neuronal survival, proliferation, differentiation, mobilization of hematopoietic stem and progenitor cells.

Product Category

Recombinant Protein / Antibody discovery

Protein

G-CSF

Synonyms

CSF3; GCSF; C17orf33; CSF3OS;

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Thr31-Pro204

Molecular Weight

The protein has a predicted MW of 44.20 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human G-CSF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

N-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human G-CSF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA (QC Test). Immobilized Human G-CSF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA (QC Test).
Human G-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human G-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human G-CSF is greater than 95% as determined by SEC-HPLC. The purity of Human G-CSF is greater than 95% as determined by SEC-HPLC.

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