Human G-CSF Protein (CSF-HM201)

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human G-CSF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Thr31-Pro204

Catalog: CSF-HM201

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Product Description	Recombinant Human G-CSF Protein is expressed from HEK293 with hFc tag at the N-terminus.It contains Thr31-Pro204.
Product Background	Granulocyte colony-stimulating factor (G-CSF) is an hemopoietic growth factor produced by fibroblasts, monocytes and endothelial cells. G-CSF is expressed by numerous cell types including neuronal, immune and endothelial cells. G-CSF is released in autocrine manner and binds to its receptor G-CSF-R which further activates numerous signaling transduction pathways including PI3K/AKT, JAK/STAT and MAP kinase, and thereby promote neuronal survival, proliferation, differentiation, mobilization of hematopoietic stem and progenitor cells.
Product Category	Recombinant Protein

Target	G-CSF
Accession	NP_757373.1
Species	Human
Amino Acid Range	Thr31-Pro204
Molecular Weight	The protein has a predicted MW of 44.20 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human G-CSF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human G-CSF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA.
	The purity of Human G-CSF is greater than 95% as determined by SEC-HPLC.
	Human G-CSF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.