• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human G-CSF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Glu25-His627
Catalog #GSR-HM101
Price
Regular price $22330
Regular price Sale price $22330
/
Size
Avantor VWR Fisher Scientific

Order by 4pm ET for delivery on:

Human G-CSF R/CD114-Protein (GSR-HM101) Summary

Product Description

 Recombinant Human G-CSF R/CD114-Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu25-His627.

Product Background

 The Granulocyte Colony-Stimulating Factor (G-CSF) receptor, a member of the hematopoietin cytokine receptor superfamily, functions as a homodimer and requires the recruitment of cytosolic protein tyrosine kinases (PTKs) to transduce its signal.

Human G-CSF R/CD114-Protein (GSR-HM101) Specifications/Details

Protein
 G-CSF R/CD114
Synonyms
 G-CSF-R; G-CSF R; CD114; G CSF R; CSF3R; Csfgr; GCSFR
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Glu25-His627
Molecular Weight
 The protein has a predicted MW of 68.29 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human G-CSF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA (QC Test).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human G-CSF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA (QC Test). Immobilized Human G-CSF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA (QC Test).
Human G-CSF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human G-CSF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human G-CSF R is greater than 95% as determined by SEC-HPLC. The purity of Human G-CSF R is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...