Human γ9δ2 TCR Protein (TCR-HM2GD)

Immobilized Human γ9δ2 TCR, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-γ9δ2 TCR Antibody, hFc Avi Tag with the EC50 of 10.6ng/ml determined by ELISA.

Immobilized Human γ9δ2 TCR, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-γδ TCR Antibody, hFc Avi Tag with the EC50 of 29.0ng/ml determined by ELISA.

Human γ9δ2 TCR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human γ9δ2 TCR is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc (IgG1)-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human γ9δ2 TCR, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-γ9δ2 TCR Antibody, hFc Avi Tag with the EC50 of 10.6ng/ml determined by ELISA.
Amino Acid Range: Ala21-Val121(TRGV9) & Ala20-Thr115(TRDV2)

Catalog #TCR-HM2GD

Price

$360⁰⁰

Size

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Human γ9δ2 TCR Protein (TCR-HM2GD) Summary

Product Description

Recombinant Human γ9δ2 TCR Protein is expressed from HEK293. Both the C-termini of the TRGV9 and TRDV2 chains are fused with a human IgG1 Fc tag and an Avi tag, respectively.It contains Ala21-Val121(TRGV9) & Ala20-Thr115(TRDV2).

Product Background

γ9δ2 T cells are a major human γδ T cell subset defined by the expression of a Vγ9Vδ2 T-cell receptor (TCR). Unlike conventional αβ T cells, they do not recognize peptide-MHC complexes but instead sense phosphoantigens via butyrophilin family members. They play key roles in cancer surveillance and anti-microbial immunity, exerting potent cytotoxic functions upon activation. Their effector maturation is accompanied by distinct phenotypic changes and modulated by natural killer receptors such as NKG2D and CD94/NKG2A. Due to their broad and MHC-independent tumor recognition, they are considered a promising target for cancer immunotherapy.

γ9δ2 Specifications

Protein	γ9δ2
Synonyms	γ9δ2 TCR
Accession	Q99603(TRGV9)&A0JD36(TRDV2)
Species	Human
Biotinylated	no
Amino Acid Range	Ala21-Val121(TRGV9) & Ala20-Thr115(TRDV2)
Molecular Weight	The protein has a predicted MW of 55.45 kDa & 56.66 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human γ9δ2 TCR, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-γ9δ2 TCR Antibody, hFc Avi Tag with the EC50 of 10.6ng/ml determined by ELISA.
Product Tag	C-hFc (IgG1)-Avi
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS, 8% trehalose (pH 7.4).
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human γ9δ2 TCR, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-γ9δ2 TCR Antibody, hFc Avi Tag with the EC50 of 10.6ng/ml determined by ELISA.
	Immobilized Human γ9δ2 TCR, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-γδ TCR Antibody, hFc Avi Tag with the EC50 of 29.0ng/ml determined by ELISA.
	Human γ9δ2 TCR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human γ9δ2 TCR is greater than 95% as determined by SEC-HPLC.