Human Galectin 3 Protein (GLT-HM103)

  • Express System: Expi293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human Galectin 3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 17.3ng/ml determined by ELISA (QC Test). The affinity constant of 33.30 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ala2-Ile250

Catalog: GLT-HM103

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Product Description Recombinant Human Galectin 3 Protein is expressed from Expi293 with His tag at the N-terminal.It contains Ala2-Ile250.
Product Category Recombinant Protein
Target Galectin 3
Synonyms AGE-R3; CBP35; GAL3; Gal-3; galactin-3; GALBPCBP35; Galectin3; GALIG; L29; L31; Lectin L-29; LGALS2; LGALS3; Mac-2; MAC2GAL3
Gene/Protein ID P17931
Species Human
Exact Sequence Ala2-Ile250
Molecular Weight The protein has a predicted MW of 27.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human Galectin 3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 17.3ng/ml determined by ELISA (QC Test). The affinity constant of 33.30 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in 50mM HEPES, 150mM NaCl, 5mM EDTA, 2mM TCEP (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Galectin 3, His Tag immobilized on CM5 Chip can bind Human LYPD3, His Tag with an affinity constant of 33.30 nM as determined in SPR assay (Biacore T200).
Immobilized Human Galectin 3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 17.3ng/ml determined by ELISA (QC Test).
Human Galectin 3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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