- Express System: HEK293
- Product Tag: C-His-Avi
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: The affinity constant of 8.30 nM as determined in SPR assay (Biacore T200). See testing image for detail.
- Amino Acid Range: His20-Leu628(GARP) & Leu21-Ser414(Latent TGF Beta 2)
Product Description |
Recombinant Human GARP&Latent TGF Beta 2 Complex Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His20-Leu628(GARP)&Leu21-Ser414(Latent TGF Beta 2). |
Product Background |
GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
GARP&Latent TGF Beta 2 Complex |
Synonyms |
Garpin; GARP; LRRC32; D11S833E; GARP&Latent TGF B; GARP&TGF B; Cetermin; G-TSF; LAP; TGF-beta-2; TGF Beta 2; GARP&Latent TGF Beta 2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
His20-Leu628(GARP) & Leu21-Ser414(Latent TGF Beta 2) |
Molecular Weight |
The protein has a predicted MW of 70.3 kDa(GARP)&45.53 kDa(Latent TGF Beta 2). Due to enzyme lysis and glycosylation, the protein migrates to 72-77 kDa(GARP)&13 kDa&46-48 kDa(Latent TGF Beta 2) based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 8.30 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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