Human GARP&Latent TGF Beta Complex Protein (GAT-HM401)
Catalog: GAT-HM401
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| Product Description | Recombinant Human GARP&Latent TGF Beta Complex Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains His20-Leu628 (GARP)&Leu30-Ser390 (Latent TGF beta). |
| Product Category | Recombinant Protein |
| Target | GARP&Latent TGF Beta Complex |
| Synonyms | LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1 |
| Gene/Protein ID | Q14392 (GARP)&P01137 (Latent TGF beta) |
| Species | Human |
| Exact Sequence | His20-Leu628 (GARP)&Leu30-Ser390 (Latent TGF beta) |
| Molecular Weight | The protein has a predicted MW of 70.3 kDa (GARP)&41.4 kDa (Latent TGF beta). Due to glycosylation, the protein migrates to 72-80 kDa (GARP)&13 kDa&42-50 kDa (Latent TGF beta) based on Tris-Bis PAGE result. |
| Activity | Immobilized Human GARP&Latent TGF Beta Complex at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GARP&Latent TGF Beta Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human GARP&Latent TGF Beta Complex at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GARP&Latent TGF Beta Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. |
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Immobilized Human GARP&Latent TGF Beta Complex at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GARP&Latent TGF Beta Antibody, hFc Tag with the EC50 of 9.9/9.1/9.3ng/ml determined by ELISA. |
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The purity of Human GARP&Latent TGF Beta Complex is greater than 95% as determined by SEC-HPLC. |
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Human GARP&Latent TGF Beta Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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