Human GARP&Latent TGF Beta 1 Complex Protein (GAT-HM401)
Human GARP&Latent TGF Beta 1 Complex Protein (GAT-HM401)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GARP&Latent TGF Beta 1 Complex, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GARP&TGF beta 1 Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: His20-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)
Catalog #GAT-HM401
Regular price $315.00
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Product Description
Recombinant Human GARP&Latent TGF Beta 1 Complex Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His20-Leu628(GARP)&Leu30-Ser390(Latent TGF beta 1).
Product Background
GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
Product Category
Recombinant Protein
Target
GARP&Latent TGF Beta 1 Complex
Synonyms
LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1
Accession
Species
Human
Biotinylated
no
Amino Acid Range
His20-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)
Molecular Weight
The protein has a predicted MW of 70.3 kDa (GARP)&41.4 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 72-80 kDa (GARP)&13 kDa&42-50 kDa (Latent TGF beta 1) based on Bis-Tris PAGE result.
Activity
Immobilized Human GARP&Latent TGF Beta 1 Complex, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GARP&TGF beta 1 Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human GARP&Latent TGF Beta 1 Complex, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GARP&TGF beta 1 Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA.
Human GARP&Latent TGF Beta 1 Complex on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human GARP&Latent TGF Beta 1 Complex is greater than 95% as determined by SEC-HPLC.

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