Human GARP&Latent TGF Beta 1 Complex Protein (GAT-HM401)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GARP&Latent TGF Beta 1 Complex, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GARP&TGF beta 1 Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: His20-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)

Catalog: GAT-HM401

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Product Description Recombinant Human GARP&Latent TGF Beta 1 Complex Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His20-Leu628(GARP)&Leu30-Ser390(Latent TGF beta 1).

Product Background

GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.

Product Category

Recombinant Protein

Target

GARP&Latent TGF Beta 1 Complex
Synonyms LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1

Gene/Protein ID

Q14392(GARP)&P01137(Latent TGF beta 1)

Species

Human

Exact Sequence

His20-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)
Molecular Weight The protein has a predicted MW of 70.3 kDa (GARP)&41.4 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 72-80 kDa (GARP)&13 kDa&42-50 kDa (Latent TGF beta 1) based on Tris-Bis PAGE result.

Activity

Immobilized Human GARP&Latent TGF Beta 1 Complex, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GARP&TGF beta 1 Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human GARP&Latent TGF Beta 1 Complex, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GARP&TGF beta 1 Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA.
The purity of Human GARP&Latent TGF Beta 1 Complex is greater than 95% as determined by SEC-HPLC.
Human GARP&Latent TGF Beta 1 Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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