Human GAS6 Protein (GAS-HM116)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GAS6, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GAS6 Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test). The affinity constant of 2.36 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ala49-Ala678

Catalog: GAS-HM116

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Product Description	Recombinant Human GAS6 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala49-Ala678.
Product Background	Growth arrest-specific 6, also known as Gas6, is a human gene encoding the Gas6 protein, which was originally found to be upregulated in growth-arrested fibroblasts. Gas6 is a member of the vitamin K-dependent family of proteins expressed in many human tissues and regulates several biological processes in cells, including proliferation, survival and migration, by binding to its receptors Tyro3, Axl and Mer (TAM).
Product Category	Recombinant Protein

Target	GAS6
Gene/Protein ID	Q14393-2
Species	Human
Exact Sequence	Ala49-Ala678
Molecular Weight	The protein has a predicted MW of 70.60 kDa. Due to glycosylation, the protein migrates to 71-75 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human GAS6, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GAS6 Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test). The affinity constant of 2.36 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human GAS6, His Tag immobilized on CM5 Chip can bind Human TYRO3, His Tag with an affinity constant of 2.36 uM as determined in SPR assay (Biacore T200).
	Immobilized Human GAS6, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GAS6 Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test).
	Immobilized Human GAS6, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Axl, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA.
	The purity of Human GAS6 is greater than 95% as determined by SEC-HPLC.
	Human GAS6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.