Product Description |
Recombinant Human GAS6 Protein is expressed from HEK293 with His tag at the C-Terminus.; It contains Ala49-Ala678. |
Product Background |
Growth arrest-specific 6, also known as Gas6, is a human gene encoding the Gas6 protein, which was originally found to be upregulated in growth-arrested fibroblasts. Gas6 is a member of the vitamin K-dependent family of proteins expressed in many human tissues and regulates several biological processes in cells, including proliferation, survival and migration, by binding to its receptors Tyro3, Axl and Mer (TAM). |
Product Category |
Recombinant Protein |
Target |
GAS6 |
Synonyms |
GAS-6; AXLLG; GAS6; DKFZp666G247; FLJ34709 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala49-Ala678 |
Molecular Weight |
The protein has a predicted MW of 70.60 kDa. Due to glycosylation, the protein migrates to 71-75 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human GAS6, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GAS6 Antibody, hFc Tag with the EC50 of 12.3 ng/ml determined by ELISA. The affinity constant of 2.36 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human GAS6, His Tag immobilized on CM5 Chip can bind Human TYRO3, His Tag with an affinity constant of 2.36 uM as determined in SPR assay (Biacore T200). |
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Immobilized Human GAS6, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GAS6 Antibody, hFc Tag with the EC50 of 12.3 ng/ml determined by ELISA. |
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Immobilized Human GAS6, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human Axl, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA (QC Test). |
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Human GAS6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human GAS6 is greater than 95% as determined by SEC-HPLC. |
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