• Express System: HEK293
  • Purity: > 95% as determined by HPLC
  • Activity: Immobilized Human GCGR VLP at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GCGR Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA.
  • Negative Control: VLP Control (VLP-HM00C)
  • Amino Acid Range: Met1-Phe477
Catalog #GCR-HM00V
Price
Regular price $1,23500
Regular price Sale price $1,23500
/
Size
Avantor VWR Fisher Scientific

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Human GCGR/Glucagon receptor VLP (GCR-HM00V) Summary

Product Description

 Recombinant Human GCGR/Glucagon receptor VLP is expressed from HEK293.It contains Met1-Phe477 (It may have cross reaction with anti-His antibody).

Product Background

 The pancreatic hormone glucagon activates the glucagon receptor (GCGR), a class B seven-transmembrane G protein-coupled receptor that couples to the stimulatory heterotrimeric G protein and provokes PKA-dependent signaling cascades vital to hepatic glucose metabolism and islet insulin secretion. The GCGR expressed at the plasma membrane is constitutively ubiquitinated and upon agonist-activation, internalized GCGRs are deubiquitinated at early endosomes and recycled via Rab4-containing vesicles.

Human GCGR/Glucagon receptor VLP (GCR-HM00V) Specifications/Details

Protein
 GCGR/Glucagon receptor
Synonyms
 Glucagon receptor; GL-R; GCGR; Glucagon R
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Met1-Phe477
Molecular Weight
 The target protein has a predicted MW of 56 kDa.
Activity
 Immobilized Human GCGR VLP at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GCGR Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA.
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22um filtered solution in PBS, 300mM L-arginine (pH 7.4). Notice: If you need it for immunization, water-soluble adjuvant is recommended.
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human GCGR VLP at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GCGR Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. Immobilized Human GCGR VLP at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GCGR Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA.
The purity of Human GCGR VLP is greater than 95% as determined by SEC-HPLC. The purity of Human GCGR VLP is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...