Human GDF15 (H202D) Protein (GDF-HE116)

Express System: E.coli
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human GDF15 (H202D), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 15.1ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ala197-Ile308 (H202D)

Catalog: GDF-HE116

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Product Description	Recombinant Human GDF15 (H202D) Protein is expressed from E.coli with His tag at the N-terminus.It contains Ala197-Ile308 (H202D).
Product Background	Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.
Product Category	Recombinant Protein

Target	GDF15 (H202D)
Gene/Protein ID	Q99988
Species	Human
Exact Sequence	Ala197-Ile308 (H202D)
Molecular Weight	The protein has a predicted MW of 13.36 kDa. The protein migrates to 15-16 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human GDF15 (H202D), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 15.1ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM HAC (pH 2.9).
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human GDF15 (H202D), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 15.1ng/ml determined by ELISA.
	Human GDF15 (H202D) on Tris-Bis PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.