Human GDF15 (H202D) Protein (GDF-HE116)
  • Express System: E.coli
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human GDF15 (H202D), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ala197-Ile308 (H202D)
Catalog #GDF-HE116
Regular price $412.50
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Product Description

Recombinant Human GDF15 (H202D) Protein is expressed from E.coli with His tag at the N-terminus.It contains Ala197-Ile308 (H202D).

Product Background

Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

GDF15

Synonyms

GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Placental TGF-β; PTGF-β; PTGFBPTGF-β

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Ala197-Ile308 (H202D)

Molecular Weight

The protein has a predicted MW of 13.36 kDa. The protein migrates to 15-16 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human GDF15 (H202D), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

N-His

Expression System

E.coli

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in 50mM HAC (pH 2.9). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human GDF15 (H202D), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test). Immobilized Human GDF15 (H202D), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test).
Immobilized Human GDF15 (H202D), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GDF15 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA. Immobilized Human GDF15 (H202D), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GDF15 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA.
Human GDF15 (H202D) on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%. Human GDF15 (H202D) on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

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support@kactusbio.us

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