Human GDF15 (H202D) Protein (GDF-HE116)
Catalog: GDF-HE116
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Product Description | Recombinant Human GDF15 (H202D) Protein is expressed from E.coli with His tag at the N-terminus.It contains Ala197-Ile308 (H202D). |
Product Background |
Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15. |
Product Category |
Recombinant Protein |
Target |
GDF15 (H202D) |
Gene/Protein ID |
Q99988 |
Species |
Human |
Exact Sequence |
Ala197-Ile308 (H202D) |
Molecular Weight | The protein has a predicted MW of 13.36 kDa. The protein migrates to 15-16 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human GDF15 (H202D), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 15.1ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His |
Express System |
E.coli |
Purity | > 95% as determined by Tris-Bis PAGE |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22 um filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM HAC (pH 2.9). |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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