- Express System: HEK293
- Product Tag: N-hFc
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Human GFRAL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human GDF15 (H202D), hFc Tag with the EC50 of 4.1ng/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Ala197-Ile308 (H202D)
Product Description |
Recombinant Human GDF15 (H202D) Protein is expressed from HEK293 with hFc tag at the N-terminus.It contains Ala197-Ile308 (H202D). |
Product Background |
Growth and differentiation factor 15 (GDF15) is an inflammation-associated hormone with poorly defined biology. Here, we investigated the role of GDF15 in bacterial and viral infections. Inflammation induced GDF15, and that GDF15 was necessary for surviving both bacterial and viral infections, as well as sepsis. The protective effects of GDF15 were largely independent of pathogen control or the magnitude of inflammatory response, suggesting a role in disease tolerance. |
Product Category |
Recombinant Protein |
Target |
GDF15 (H202D) |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala197-Ile308 (H202D) |
Molecular Weight |
The protein has a predicted MW of 37.88 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human GFRAL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human GDF15 (H202D), hFc Tag with the EC50 of 4.1ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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