Product Description |
Recombinant Human GDF15 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ala197-Ile308. |
Product Background |
Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15. |
Product Category |
Recombinant Protein |
Target |
GDF15 |
Synonyms |
GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Placental TGF-β; PTGF-β; PTGFBPTGF-β |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala197-Ile308 |
Molecular Weight |
The protein has a predicted MW of 13.5 kDa. The protein migrates to 15-16 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human GDF15, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 11.5 ng/ml determined by ELISA (QC Test). The affinity constant of 0.014 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-His |
Express System |
E.coli |
Purity |
>95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50 mM HAc (pH 2.9). |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF15, His Tag with an affinity constant of 0.014 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human GDF15, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 11.5 ng/ml determined by ELISA (QC Test). |
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Human GDF15 on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%. |
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Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8 ng/ml. |
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