Human GDF15 Protein (GDF-HE115)

  • Express System: E.coli
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human GDF15, His Tag at 0.5_g/ml (100_l/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 22.8ng/ml determined by ELISA. The affinity constant of 0.014 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ala197-Ile308

Catalog: GDF-HE115

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Product Description Recombinant Human GDF15 Protein is expressed from E.coli with His tag at the N-terminal.It contains Ala197-Ile308.
Product Category Recombinant Protein
Target GDF15
Synonyms GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta
Gene/Protein ID Q99988-1
Species Human
Exact Sequence Ala197-Ile308
Molecular Weight The protein has a predicted MW of 13.5 kDa. The protein migrates to 15-16 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human GDF15, His Tag at 0.5_g/ml (100_l/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 22.8ng/ml determined by ELISA. The affinity constant of 0.014 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-His
Express System E.coli
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in 50mM HAc (pH 2.9).
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF15, His Tag with an affinity constant of 0.014 nM as determined in SPR assay (Biacore T200).
Immobilized Human GDF15, His Tag at 0.5_g/ml (100_l/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 22.8ng/ml determined by ELISA.
Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8ng/ml.
Human GDF15 on Tris-Bis PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

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