Name: Human GDF15 Protein (GDF-HE115)
Brand: KactusBio
SKU: GDF-HE115-100UG
Price: 315.0 USD
Availability: InStock

Express System: E.coli
Product Tag: N-His
Purity: >95% as determined by Bis-Tris PAGE
Activity: Immobilized Human GDF15, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 13.1 ng/ml determined by ELISA (QC Test). The affinity constant of 0.014 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ala197-Ile308

Catalog #GDF-HE115

Size

100UG

500UG

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Product Description	Recombinant Human GDF15 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ala197-Ile308.
Product Background	Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.
Product Category	Recombinant Protein

Protein	GDF15
Synonyms	GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Placental TGF-β; PTGF-β; PTGFBPTGF-β
Accession	Q99988-1
Species	Human
Biotinylated	no
Amino Acid Range	Ala197-Ile308
Molecular Weight	The protein has a predicted MW of 13.5 kDa. The protein migrates to 15-16 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human GDF15, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 13.1 ng/ml determined by ELISA (QC Test). The affinity constant of 0.014 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Expression System	E.coli
Purity	>95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50 mM HAc (pH 2.9).
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF15, His Tag with an affinity constant of 0.014 nM as determined in SPR assay (Biacore T200).
	Human GDF15 on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.
	Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8 ng/ml.