Human GDF15 Protein (GDF-HM215)
Catalog: GDF-HM215
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| Product Description | Recombinant Human GDF15 Protein is expressed from Expi293 with hFc tag at the N-terminal.It contains Ala197-Ile308. |
| Product Category | Recombinant Protein |
| Target | GDF15 |
| Synonyms | GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta |
| Gene/Protein ID | Q99988 |
| Species | Human |
| Exact Sequence | Ala197-Ile308 |
| Molecular Weight | The protein has a predicted MW of 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Biotinylated Human GFRAL, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human GFD15, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | N-hFc |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 92% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Biotinylated Human GFRAL, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human GFD15, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. |
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The purity of Human GDF15 is greater than 92% as determined by SEC-HPLC. |
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Human GDF15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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