Name: Human GFR alpha 1 Protein (GFR-HM11A)
Brand: KactusBio
SKU: GFR-HM11A-100UG
Price: 231.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GDNF, No Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Asp25-Ser424

Catalog #GFR-HM11A

Size

100UG

500UG

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Product Description	Recombinant Human GFR alpha 1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp25-Ser424.
Product Background	Neuroblastomas often undergo spontaneous differentiation and/or regression in vivo, which is at least partly regulated by the signals through neurotrophins and their receptors. Recently, glial cell line-derived neurotrophic factor (GDNF) and a second family member, neurturin (NTN), have been found to mediate their signals by binding to a heterotetrameric complex of c-Ret tyrosine kinase receptors and glycosylphosphatidylinositol-linked proteins, GFR alpha-1 (GDNFR-alpha) or GFR alpha-2 (TrnR2/GDNFR-beta/NTNR-alpha/RETL2).
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	GFR alpha 1
Synonyms	GDNFRA; GFRA1; GDNFR; TRNR1; RET1L; GDNF R alpha-1; GDNFRMGC23045; GFR alpha1; GFR alpha-1; RET ligand 1; RETL1FLJ10561
Accession	NP_665736.1
Species	Human
Biotinylated	no
Amino Acid Range	Asp25-Ser424
Molecular Weight	The protein has a predicted MW of 45.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human GDNF, No Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human GDNF, No Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA.
	Human GFR alpha 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human GFR alpha 1 is greater than 95% as determined by SEC-HPLC.