Human GFR alpha 1 Protein (GFR-HM11A)
Catalog: GFR-HM11A
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| Product Description | Recombinant Human GFR alpha 1 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Asp25-Ser424. |
| Product Category | Recombinant Protein |
| Target | GFR alpha 1 |
| Synonyms | GDNFRA; GFRA1; GDNFR; TRNR1; RET1L; GDNF R alpha-1; GDNFRMGC23045; GFR alpha1; GFR alpha-1; RET ligand 1; RETL1FLJ10561 |
| Gene/Protein ID | NP_665736.1 |
| Species | Human |
| Exact Sequence | Asp25-Ser424 |
| Molecular Weight | The protein has a predicted MW of 45.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human GDNF, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 6.0ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human GDNF, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 6.0ng/ml determined by ELISA. |
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The purity of Human GFR alpha 1 is greater than 95% as determined by SEC-HPLC. |
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Human GFR alpha 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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