Name: Human GFR alpha 1 Protein (GFR-HM11A)
Brand: KactusBio
SKU: GFR-HM11A-100UG
Price: 210.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GDNF, No Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Asp25-Ser424

Catalog #GFR-HM11A

Size

100UG

500UG

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Product Description	Recombinant Human GFR alpha 1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp25-Ser424.
Product Background	Neuroblastomas often undergo spontaneous differentiation and/or regression in vivo, which is at least partly regulated by the signals through neurotrophins and their receptors. Recently, glial cell line-derived neurotrophic factor (GDNF) and a second family member, neurturin (NTN), have been found to mediate their signals by binding to a heterotetrameric complex of c-Ret tyrosine kinase receptors and glycosylphosphatidylinositol-linked proteins, GFR alpha-1 (GDNFR-alpha) or GFR alpha-2 (TrnR2/GDNFR-beta/NTNR-alpha/RETL2).
Product Category	Recombinant Protein / Antibody discovery

Protein	GFR alpha 1
Synonyms	GDNFRA; GFRA1; GDNFR; TRNR1; RET1L; GDNF R alpha-1; GDNFRMGC23045; GFR alpha1; GFR alpha-1; RET ligand 1; RETL1FLJ10561
Accession	NP_665736.1
Species	Human
Biotinylated	no
Amino Acid Range	Asp25-Ser424
Molecular Weight	The protein has a predicted MW of 45.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human GDNF, No Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human GDNF, No Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA.
	Human GFR alpha 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human GFR alpha 1 is greater than 95% as determined by SEC-HPLC.