Human GFR alpha 1 Protein (GFR-HM11A)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GDNF, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 6.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp25-Ser424

Catalog: GFR-HM11A

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Product Description Recombinant Human GFR alpha 1 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Asp25-Ser424.
Product Category Recombinant Protein
Target GFR alpha 1
Synonyms GDNFRA; GFRA1; GDNFR; TRNR1; RET1L; GDNF R alpha-1; GDNFRMGC23045; GFR alpha1; GFR alpha-1; RET ligand 1; RETL1FLJ10561
Gene/Protein ID NP_665736.1
Species Human
Exact Sequence Asp25-Ser424
Molecular Weight The protein has a predicted MW of 45.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human GDNF, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 6.0ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human GDNF, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 6.0ng/ml determined by ELISA.
The purity of Human GFR alpha 1 is greater than 95% as determined by SEC-HPLC.
Human GFR alpha 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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