Human GFRA3 Protein (GFR-HM1A3)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GFRA3, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-GFRA3 Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA (QC Test). See testing image for detail.
Exact Sequence: Asp32-Trp382

Catalog: GFR-HM1A3

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Product Description	Recombinant Human GFRA3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp32-Trp382.
Product Background	GDNF (glial-cell-line derived neurotrophic factor) is a potent neurotrophic factor for dopaminergic neurons. Neuropsychiatric diseases and their treatments are associated with alterations in the levels of both GDNF and its receptor family (GDNF family receptor alpha or GFRA). GFRA1, GFRA2 and GFRA3 are located in chromosomal regions with suggestive linkage to schizophrenia.
Product Category	Recombinant Protein

Target	GFRA3
Synonyms	GDNFR-alpha-3; GFR-alpha-3; GFRA3; GFR alpha3; GPI-linked receptor
Gene/Protein ID	O60609-1
Species	Human
Exact Sequence	Asp32-Trp382
Molecular Weight	The protein has a predicted MW of 40.3 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human GFRA3, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-GFRA3 Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human GFRA3, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-GFRA3 Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA (QC Test).
	Immobilized Human GFRA3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA.
	The purity of Human GFRA3 is greater than 95% as determined by SEC-HPLC.
	Human GFRA3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.