Human GFRAL/GFR alpha-like Protein (GFL-HM201)

Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF15, His Tag with an affinity constant of 0.014 nM as determined in SPR assay (Biacore T200).

Immobilized Human GDF15, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 13.1ng/ml determined by ELISA (QC Test).

Human GFRAL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of- Human GFRAL is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GDF15, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 13.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.014 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Ser19-Glu351

Catalog #GFL-HM201

Price

$346⁵⁰

Size

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Human GFRAL/GFR alpha-like Protein (GFL-HM201) Summary

Product Description

Recombinant Human GFRAL/GFR alpha-like Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ser19-Glu351.

Product Background

GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.

Human GFRAL/GFR alpha-like Protein (GFL-HM201) Specifications/Details

Protein	GFRAL/GFR alpha-like
Synonyms	GFR alpha-like; GFRAL; GRAL; C6orf144
Accession	Q6UXV0
Species	Human
Biotinylated	no
Amino Acid Range	Ser19-Glu351
Molecular Weight	The protein has a predicted MW of 64.6 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human GDF15, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 13.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.014 nM as determined in SPR assay (Biacore T200).
Product Tag	C-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF15, His Tag with an affinity constant of 0.014 nM as determined in SPR assay (Biacore T200).
	Immobilized Human GDF15, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 13.1ng/ml determined by ELISA (QC Test).
	Human GFRAL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of- Human GFRAL is greater than 95% as determined by SEC-HPLC.