Human GFRAL/GFR alpha-like Protein (GFL-HM201)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GDF15, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.014 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ser19-Glu351
Catalog #GFL-HM201
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Product Description
 Recombinant Human GFRAL/GFR alpha-like Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser19-Glu351.
Product Background
 GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
Product Category
 Recombinant Protein
Target
 GFRAL/GFR alpha-like
Synonyms
 GFR alpha-like; GFRAL; GRAL; C6orf144
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ser19-Glu351
Molecular Weight
 The protein has a predicted MW of 64.6 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human GDF15, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.014 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF15, His Tag with an affinity constant of 0.014 nM as determined in SPR assay (Biacore T200).
Immobilized Human GDF15, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA (QC Test).
Human GFRAL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of- Human GFRAL is greater than 95% as determined by SEC-HPLC.

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