Human GFRAL/GFR alpha-like Protein (GFL-HM201)
Catalog: GFL-HM201
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Product Description | Recombinant Human GFRAL/GFR alpha-like Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Ser19-Glu351. |
Product Category | Recombinant Protein |
Target | GFRAL/GFR alpha-like |
Synonyms | GFR alpha-like; GFRAL; GRAL; C6orf144 |
Gene/Protein ID | Q6UXV0 |
Species | Human |
Exact Sequence | Ser19-Glu351 |
Molecular Weight | The protein has a predicted MW of 64.6 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human GDF15, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 15ng/ml determined by ELISA (QC Test). The affinity constant of 0.014 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag | C-hFc |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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