Human GFRAL/GFR alpha-like Protein (GFL-HM401)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GFRAL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human GDF15, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ser19-Glu351

Catalog: GFL-HM401

Order now get it on

Bulk Quote | Test Sample

Product Description	Recombinant Human GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser19-Glu351.
Product Background	GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
Product Category	Recombinant Protein

Target	GFRAL/GFR alpha-like
Synonyms	GFR alpha-like; GFRAL; GRAL; C6orf144
Gene/Protein ID	Q6UXV0
Species	Human
Exact Sequence	Ser19-Glu351
Molecular Weight	The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 48-62 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human GFRAL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human GDF15, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4).Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human GFRAL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human GDF15, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA.
	The purity of-Human GFRAL is greater than 95% as determined by SEC-HPLC.
	Human GFRAL on Tris-Bis PAGE under reduced condition.The purity is greater than 95%.