Human GFRAL/GFR alpha-like Protein (GFL-HM401)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GFRAL, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human GDF15, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser19-Glu351

Catalog: GFL-HM401

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Product Description Recombinant Human GFRAL/GFR alpha-like Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Ser19-Glu351.
Product Category Recombinant Protein
Target GFRAL/GFR alpha-like
Synonyms GFR alpha-like; GFRAL; GRAL; C6orf144
Gene/Protein ID Q6UXV0
Species Human
Exact Sequence Ser19-Glu351
Molecular Weight The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 48-62 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human GFRAL, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human GDF15, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human GFRAL, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human GDF15, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA.
The purity of Human GFRAL is greater than 95% as determined by SEC-HPLC.
Human GFRAL on Tris-Bis PAGE under reduced condition.The purity is greater than 95%.

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