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Human GGT1 Protein (GGT-HM101) Summary
Product Description |
Recombinant Human GGT1 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Pro27-Tyr569. |
Product Background |
Gamma-glutamyl transferase (GGT; EC 2.3.2.2) is the only enzyme capable of degrading glutathione (GSH) in extra-cytosolic spaces. Overexpression of γ-glutamyl transpeptidase (GGT1) has been implicated in an array of human diseases including asthma, reperfusion injury, and cancer. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human GGT1 Protein (GGT-HM101) Specifications/Details
Protein |
GGT1 |
Synonyms |
Gamma-glutamyltransferase 1; GGT 1; CD224; GGT; GGT-1; GGTD; D22S672; D22S73; D22S732 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Pro27-Tyr569 |
Molecular Weight |
The protein has a predicted MW of 59.69 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 55-65 kDa (heavy chain) and 25-30 kDa (light chain) based on Bis-Tris PAGE result. |
Product Tag |
N-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human GGT1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human GGT1 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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