Human GIP Protein (GIP-HM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GIP, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GIP Antibody, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Glu22-Gln93

Catalog: GIP-HM101

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Product Description Recombinant Human GIP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu22-Gln93.

Product Background

The potential application of glucose-dependent insulinotropic polypeptide (gastric inhibitory polypeptide, GIP) in the management of obesity and type 2 diabetes has been controversial. Initial interest in the therapeutic use of GIP was dampened by evidence that its insulinotropic activity was reduced in type 2 diabetes and by reports that it increased glucagon secretion and adipose deposition in non-diabetic individuals.

Product Category

Recombinant Protein

Target

GIP
Synonyms GIP; Incretin
Accession

Species

Human

Amino Acid Range

Glu22-Gln93
Molecular Weight The protein has a predicted MW of 9.2 kDa. Due to glycosylation, the protein migrates to 15-25 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human GIP, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GIP Antibody, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human GIP, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GIP Antibody, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA.
The purity of Human GIP is greater than 95% as determined by SEC-HPLC.
Human GIP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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