Human GITR/TNFRSF18 Protein (GTR-HM101)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GITR, His Tag at 1μg/ml (100μl/Well). Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 0.2μg/ml determined by ELISA. The affinity constant of 4.76 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln26-Glu161

Catalog: GTR-HM101

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Product Description Recombinant Human GITR/TNFRSF18 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Gln26-Glu161.
Product Category Recombinant Protein
Target GITR/TNFRSF18
Synonyms CD357; GITR; GITR-D; TNFRSF18; AITR
Gene/Protein ID Q9Y5U5-1
Species Human
Exact Sequence Gln26-Glu161
Molecular Weight The protein has a predicted MW of 15.4 kDa. Due to glycosylation, the protein migrates to 25-28 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human GITR, His Tag at 1μg/ml (100μl/Well). Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 0.2μg/ml determined by ELISA. The affinity constant of 4.76 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human GITR, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human GITR Ligand Trimer, hFc Tag with an affinity constant of 4.76 nM as determined in SPR assay (Biacore T200).
Immobilized Human GITR, His Tag at 1μg/ml (100μl/Well). Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 0.2μg/ml determined by ELISA.
Immobilized Human GITR, His Tag at 0.5μg (100μl/Well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA.
The purity of Human GITR is greater than 95% as determined by SEC-HPLC.
Human GITR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%

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