Human GITR/TNFRSF18 Protein (GTR-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GITR Ligand Trimer, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, His Tag with the EC50 of 2.60ug/ml determined by ELISA (QC Test). The affinity constant of 4.47 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln26-Glu161

Catalog: GTR-HM101

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Product Description	Recombinant Human GITR/TNFRSF18 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln26-Glu161.
Product Background	GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation.GIRT is a receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
Product Category	Recombinant Protein

Target	GITR/TNFRSF18
Synonyms	CD357; GITR; GITR-D; TNFRSF18; AITR
Accession	Q9Y5U5-1
Species	Human
Amino Acid Range	Gln26-Glu161
Molecular Weight	The protein has a predicted MW of 15.4 kDa. Due to glycosylation, the protein migrates to 25-28 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human GITR Ligand Trimer, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, His Tag with the EC50 of 2.60ug/ml determined by ELISA (QC Test). The affinity constant of 4.47 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human GITR, His Tag captured on CM5 Chip via Anti-his antibody can bind Human GITR Ligand Trimer, hFc Tag with an affinity constant of 4.47 nM as determined in SPR assay (Biacore T200).
	Immobilized Human GITR Ligand Trimer, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, His Tag with the EC50 of 2.60ug/ml determined by ELISA (QC Test).
	Immobilized Human GITR, His Tag at 0.5ug (100ul/Well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 27.9ng/ml determined by ELISA.
	The purity of Human GITR is greater than 95% as determined by SEC-HPLC.
	Human GITR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%