• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GITR Ligand Trimer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, hFc Tag with the EC50 of 50.8ng/ml determined by ELISA.
  • Amino Acid Range: Gln26-Glu161
Catalog #GTR-HM201
Price
Regular price $29700
Regular price Sale price $29700
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Size
Avantor VWR Fisher Scientific

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Human GITR/TNFRSF18 Protein (GTR-HM201) Summary

Product Description

 Recombinant Human GITR/TNFRSF18 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gln26-Glu161.

Product Background

 GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation.GIRT is a receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.

Human GITR/TNFRSF18 Protein (GTR-HM201) Specifications/Details

Protein
 GITR/TNFRSF18
Synonyms
 CD357; GITR; GITR-D; TNFRSF18; AITR
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gln26-Glu161
Molecular Weight
 The protein has a predicted MW of 41.3 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human GITR Ligand Trimer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, hFc Tag with the EC50 of 50.8ng/ml determined by ELISA.
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human GITR Ligand Trimer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, hFc Tag with the EC50 of 50.8ng/ml determined by ELISA. Immobilized Human GITR Ligand Trimer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, hFc Tag with the EC50 of 50.8ng/ml determined by ELISA.
Human GITR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human GITR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human GITR is greater than 95% as determined by SEC-HPLC. The purity of Human GITR is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...