Human GLP-1R Protein (GLP-HM30R) Summary
Product Description |
Recombinant Human GLP-1R Protein is expressed from HEK293 with mFc (IgG1) tag at the C-terminus.It contains Arg24-Glu139. |
Product Background |
Glucagon-like peptide-1 (GLP-1) is produced by the gut, stimulates insulin secretion from the pancreatic β-cells, and inhibits glucagon secretion from the α-cells. The GLP-1 receptor (GLP-1R) agonists are used in the treatment of type 2 diabetes (T2DM). |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human GLP-1R Protein (GLP-HM30R) Specifications/Details
Protein |
GLP-1R |
Synonyms |
GLP1R; GLP-1-R; GLP-1R; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Arg24-Glu139 |
Molecular Weight |
The protein has a predicted MW of 39.20 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human GLP-1R, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GLP-1R Antibody, hFc Tag with the EC50 of 17.0ng/ml determined by ELISA. |
Product Tag |
C-mFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human GLP-1R, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GLP-1R Antibody, hFc Tag with the EC50 of 17.0ng/ml determined by ELISA. |
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Human GLP-1R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human GLP-1R is greater than 95% as determined by SEC-HPLC. |
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