Human Glypican 1/GPC1 Protein (GPC-HM111)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human FGF basic at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 12.6ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Asp24-Ser530

Catalog: GPC-HM111

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Product Description	Recombinant Human Glypican 1/GPC1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp24-Ser530.
Product Background	CAR-T cells targeting glypican-1 (GPC1)-specific human and murine CAR-T cells generated from our original anti-human/mouse GPC1 antibody showed strong antitumor effects in xenogeneic and syngeneic mouse models, respectively. Importantly, the murine CAR-T cells enhanced endogenous T cell responses against a non-GPC1 tumor antigen through the mechanism of antigen-spreading and showed synergistic antitumor effects with anti-PD-1 antibody without any adverse effects in syngeneic models.
Product Category	Recombinant Protein

Target	Glypican 1/GPC1
Synonyms	Glypican-1; FLJ38078; GPC1
Gene/Protein ID	P35052-1
Species	Human
Exact Sequence	Asp24-Ser530
Molecular Weight	The protein has a predicted MW of 57.1 kDa. Due to glycosylation, the protein migrates to 70-135 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human FGF basic at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 12.6ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human FGF basic at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 12.6ng/ml determined by ELISA.
	Immobilized Human Glypican 1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Glypican 1 Antibody, hFc Tag with the EC50 of 21.9ng/ml determined by ELISA (QC Test).
	The purity of Human Glypican 1 is greater than 95% as determined by SEC-HPLC.
	Human Glypican 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.