Human GM-CSF Protein (GSF-HE001)

  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GM-CSF at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA. The affinity constant of 0.79nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ala18-Glu144

Catalog: GSF-HE001

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Product Description Recombinant Human GM-CSF Protein is expressed from E.coli without tag.It contains Ala18-Glu144.
Product Category Recombinant Protein
Target GM-CSF
Synonyms CSF; CSF2; GMCSF; GM-CSF; MGC131935; MGC138897
Gene/Protein ID P04141
Species Human
Exact Sequence Ala18-Glu144
Molecular Weight The protein has a predicted MW of 14.6 kDa same as Tris-Bis PAGE result.
Activity Immobilized Human GM-CSF at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA. The affinity constant of 0.79nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag No Tag
Express System E.coli
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human GM-CSF R alpha, His Tag captured on CM5 Chip via anti-His antibody can bind Human GM-CSF, No Tag with an affinity constant of 0.79nM as determined in SPR assay (Biacore T200).
Immobilized Human GM-CSF at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA.
The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 0.1 ng/mL.
The purity of Human GM-CSF is greater than 95% as determined by SEC-HPLC.
Human GM-CSF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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