Human GM-CSF Protein (GSF-HE001)
Catalog: GSF-HE001
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Product Description | Recombinant Human GM-CSF Protein is expressed from E.coli without tag.It contains Ala18-Glu144. |
Product Category | Recombinant Protein |
Target | GM-CSF |
Synonyms | CSF; CSF2; GMCSF; GM-CSF; MGC131935; MGC138897 |
Gene/Protein ID | P04141 |
Species | Human |
Exact Sequence | Ala18-Glu144 |
Molecular Weight | The protein has a predicted MW of 14.6 kDa same as Tris-Bis PAGE result. |
Activity | Immobilized Human GM-CSF at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA. The affinity constant of 0.79nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag | No Tag |
Express System | E.coli |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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