Human GM-CSF Protein (GSF-HE001)
  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GM-CSF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.79nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ala18-Glu144
Catalog #GSF-HE001
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Product Description
 Recombinant Human GM-CSF Protein is expressed from E.coli without tag.It contains Ala18-Glu144.
Product Background
 Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim.
Product Category
 Recombinant Protein
Target
 GM-CSF
Synonyms
 CSF; CSF2; GMCSF; GM-CSF; MGC131935; MGC138897
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala18-Glu144
Molecular Weight
 The protein has a predicted MW of 14.6 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Human GM-CSF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.79nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 No Tag
Express System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human GM-CSF R alpha, His Tag captured on CM5 Chip via anti-His antibody can bind Human GM-CSF, No Tag with an affinity constant of 0.79nM as determined in SPR assay (Biacore T200).
Immobilized Human GM-CSF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA (QC Test).
Human GM-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 0.02-0.1 ng/mL (QC Test).
The purity of Human GM-CSF is greater than 95% as determined by SEC-HPLC.
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