• Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GM-CSF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.79nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Ala18-Glu144
Catalog #GSF-HE001
Price
Regular price $24750
Regular price Sale price $24750
/
Size
Avantor VWR Fisher Scientific

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Human GM-CSF Protein (GSF-HE001) Summary

Product Description

 Recombinant Human GM-CSF Protein is expressed from E.coli without tag.It contains Ala18-Glu144.

Product Background

 Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim.

Human GM-CSF Protein (GSF-HE001) Specifications/Details

Protein
 GM-CSF
Synonyms
 CSF; CSF2; GMCSF; GM-CSF; MGC131935; MGC138897;Colony stimulating factor-2; Colony stimulating factor 2
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala18-Glu144
Molecular Weight
 The protein has a predicted MW of 14.6 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Human GM-CSF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.79nM as determined in SPR assay (Biacore T200).
Product Tag
 No Tag
Fluorophore
 Unconjugated
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human GM-CSF R alpha, His Tag captured on CM5 Chip via anti-His antibody can bind Human GM-CSF, No Tag with an affinity constant of 0.79nM as determined in SPR assay (Biacore T200). Human GM-CSF R alpha, His Tag captured on CM5 Chip via anti-His antibody can bind Human GM-CSF, No Tag with an affinity constant of 0.79nM as determined in SPR assay (Biacore T200).
Immobilized Human GM-CSF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA (QC Test). Immobilized Human GM-CSF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA (QC Test).
Human GM-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human GM-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 0.02-0.1 ng/mL (QC Test). The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 0.02-0.1 ng/mL (QC Test).
The purity of Human GM-CSF is greater than 95% as determined by SEC-HPLC. The purity of Human GM-CSF is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...