Human GM-CSF Protein (GSF-HE001)

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GM-CSF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.79nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ala18-Glu144

Catalog: GSF-HE001

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Product Description	Recombinant Human GM-CSF Protein is expressed from E.coli without tag.It contains Ala18-Glu144.
Product Background	Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim.
Product Category	Recombinant Protein

Target	GM-CSF
Synonyms	CSF; CSF2; GMCSF; GM-CSF; MGC131935; MGC138897
Gene/Protein ID	P04141
Species	Human
Exact Sequence	Ala18-Glu144
Molecular Weight	The protein has a predicted MW of 14.6 kDa same as Tris-Bis PAGE result.
Activity	Immobilized Human GM-CSF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.79nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	No Tag
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human GM-CSF R alpha, His Tag captured on CM5 Chip via anti-His antibody can bind Human GM-CSF, No Tag with an affinity constant of 0.79nM as determined in SPR assay (Biacore T200).
	Immobilized Human GM-CSF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test).
	The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 0.02-0.1 ng/mL (QC Test).
	The purity of Human GM-CSF is greater than 95% as determined by SEC-HPLC.
	Human GM-CSF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.