Human GM-CSF R alpha Protein (GSF-HM1RA)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GM-CSF R alpha, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GM-CSF R alpha Antibody, hFc Tag with the EC50 of 15.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.79nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Glu23-Gly320

Catalog: GSF-HM1RA

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Product Description Recombinant Human GM-CSF R alpha Protein is expressed from Expi293 with His tag at the C-terminal.It contains Glu23-Gly320.
Product Category Recombinant Protein
Target GM-CSF R alpha
Synonyms GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha; CSF2R; CSF2RY; CSF2RA; CDw116; CD116; CSF2RAX; CSF2RAY; CSF2RX; GMCSFR; GMR; SMDP4
Gene/Protein ID P15509-1
Species Human
Exact Sequence Glu23-Gly320
Molecular Weight The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human GM-CSF R alpha, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GM-CSF R alpha Antibody, hFc Tag with the EC50 of 15.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.79nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human GM-CSF R alpha , His Tag captured on CM5 Chip via anti-His antibody can bind Human GM-CSF, No Tag with an affinity constant of 0.79nM as determined in SPR assay (Biacore T200).
Immobilized Human GM-CSF R alpha, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GM-CSF R alpha Antibody, hFc Tag with the EC50 of 15.8ng/ml determined by ELISA (QC Test).
Immobilized Human GM-CSF, No Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human GM-CSF R alpha, His Tag with the EC50 of 1.0μg/ml determined by ELISA.
The purity of Human GM-CSF R alpha is greater than 95% as determined by SEC-HPLC.
Human GM-CSF R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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