Product Description |
Recombinant Human GM-CSF R alpha Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu23-Gly320. |
Product Background |
In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua. |
Product Category |
Recombinant Protein |
Target |
GM-CSF R alpha |
Synonyms |
GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha; CSF2R; CSF2RY; CSF2RA; CDw116; CD116; CSF2RAX; CSF2RAY; CSF2RX; GMCSFR; GMR; SMDP4 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu23-Gly320 |
Molecular Weight |
The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human GM-CSF, No Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human GM-CSF R alpha, His Tag with the EC50 of 1.0ug/ml determined by ELISA. The affinity constant of 0.79nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human GM-CSF R alpha , His Tag captured on CM5 Chip via anti-His antibody can bind Human GM-CSF, No Tag with an affinity constant of 0.79nM as determined in SPR assay (Biacore T200). |
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Immobilized Human GM-CSF, No Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human GM-CSF R alpha, His Tag with the EC50 of 1.0ug/ml determined by ELISA. |
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Immobilized Human GM-CSF R alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GM-CSF R alpha Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA (QC Test). |
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Human GM-CSF R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human GM-CSF R alpha is greater than 95% as determined by SEC-HPLC. |
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