Size-exclusion chromatography (SEC) analysis of recombinant protein, showing a single peak at ~8.75 minutes, indicating high purity and monodispersity.
Human HLA-A*24:02&B2M&GP100 Intron 4 (VYFFLPDHL) Monomer Protein (MHC-HM433)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly25-Thr305(HLA-A*24:02), Ile21-Met119(B2M) and VYFFLPDHL peptide
Catalog #MHC-HM433
Regular price $341.00
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Human HLA-A*24:02&B2M&GP100 Intron 4 (VYFFLPDHL) Monomer Protein (MHC-HM433) Summary

Product Description

Recombinant Human HLA-A*24:02&B2M&GP100 Intron 4 (VYFFLPDHL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*24:02), Ile21-Met119(B2M) and VYFFLPDHL peptide.

Product Background

Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of- the MHC Class I, B2M and YLEPGPVTA peptide of- the gp100.

Product Category

Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

HLA-A*24:02&B2M&GP100 Intron 4 (VYFFLPDHL) Specifications

Protein
HLA-A*24:02&B2M&GP100 Intron 4 (VYFFLPDHL)
Synonyms
HLA-A*24:02; HLA-A2402; MHC I; gp100; Glycoprotein 100; ME20-M; P100; GP100 Intron 4; Intron 4

MHC Form

Monomer

Allele

HLA-A*24:02
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gly25-Thr305(HLA-A*24:02), Ile21-Met119(B2M) and VYFFLPDHL peptide
Molecular Weight
The protein has a predicted MW of 50.40 kDa. Due to glycosylation, the protein migrates to 55-63 kDa based on Bis-Tris PAGE result.
Product Tag
C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human HLA-A*24:02&B2M&GP100 Intron 4 (VYFFLPDHL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human HLA-A*24:02&B2M&GP100 Intron 4 (VYFFLPDHL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HLA-A*24:02&B2M&GP100 Intron 4 (VYFFLPDHL) Monomer is greater than 95% as determined by SEC-HPLC. The purity of Human HLA-A*24:02&B2M&GP100 Intron 4 (VYFFLPDHL) Monomer is greater than 95% as determined by SEC-HPLC.
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