Product Description |
Recombinant Human gp130/CD130/IL-6 R beta-Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu23-Glu619. |
Product Background |
gp130 is a common signal transducing component of the functional receptor complexes for the interleukin (IL)-6 family of cytokines, ie, IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M, ciliary neurotrophic factor, and cardiotrophin-1. These cytokines exhibit pleiotropic biological activities in, for instance, immune, hematopoietic, and neural systems, and function in a redundant manner owing to the shared usage of gp130. |
Product Category |
Recombinant Protein |
Protein |
gp130/CD130/IL-6 R beta |
Synonyms |
gp130; CD130; IL-6 R beta; IL-6R-beta; IL-6RB; CDw130; IL6ST |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu23-Glu619 |
Molecular Weight |
The protein has a predicted MW of 68.94 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-6 R alpha, hFc Tag at 2ug/ml (100ul/well) on the Human IL-6, No Tag precoated plate (5ug/ml). Dose response curve for Human gp130, His Tag with the EC50 of 49.6ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Immobilized Human IL-6 R alpha, hFc Tag at 2ug/ml (100ul/well) on the Human IL-6, No Tag precoated plate (5ug/ml). Dose response curve for Human gp130, His Tag with the EC50 of 49.6ng/ml determined by ELISA (QC Test). |
|
|
Human gp130 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human gp130 is greater than 95% as determined by SEC-HPLC. |
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