SEC-HPLC chromatogram showing two peaks at approximately 5.9 and 8.6 minutes, indicating the presence of both aggregate and monomeric forms of the protein sample.
Human GP1BB Protein (GP1-HM2BB)
SEC-HPLC chromatogram showing two peaks at approximately 5.9 and 8.6 minutes, indicating the presence of both aggregate and monomeric forms of the protein sample.
Human GP1BB Protein (GP1-HM2BB)
  • Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Pro27-Cys147
Catalog #GP1-HM2BB
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Size
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Human GP1BB Protein (GP1-HM2BB) Summary

Product Description

 Recombinant Human GP1BB Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Pro27-Cys147.

Product Background

 The glycoprotein (GP) Ib-IX complex is the receptor on platelet surfaces that mediates their adhesion to subendothelium. It comprises three polypeptides (GP Ib alpha, GP Ib beta, GP IX), each of which belongs to a superfamily of proteins containing conserved leucine-rich motifs. Association between GP Ib alpha and GP Ib beta was demonstrated biochemically on immunoblots of detergent lysates of CHO alpha beta cells; electrophoresis under nonreducing conditions revealed the two subunits to be covalently linked through a disulfide bond.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human GP1BB Protein (GP1-HM2BB) Specifications/Details

Protein
 GP1BB
Synonyms
 GP-Ib beta; GPIb-beta; GPIbB; Gp1bb; BDPLT1; BS; CD42c; GP1BB; GPIbb; GPIbbeta;GP-Ib β; GPIb-β; GPIbβ
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Pro27-Cys147
Molecular Weight
 The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 47-52 kDa based on Bis-Tris PAGE result.
Product Tag
 C-hFc (IgG1)

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human GP1BB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human GP1BB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human GP1BB is greater than 95% as determined by SEC-HPLC. The purity of Human GP1BB is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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