Human GPA34/VSIG1 Protein (GPA-HM234)

Immobilized Human GPA34, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPA34 Antibody, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA.

Human GPA34 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human GPA34 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GPA34, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPA34 Antibody, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA.
Amino Acid Range: Val22-Glu232

Catalog #GPA-HM234

Price

$315⁰⁰

Size

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Human GPA34/VSIG1 Protein (GPA-HM234) Summary

Product Description

Recombinant Human GPA34/VSIG1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Val22-Glu232.

Product Background

VSIG1, a cell adhesion protein of the immunoglobulin superfamily, is preferentially expressed in stomach, testis, and certain gastric, esophageal and ovarian cancers. Here, we describe the expression patterns of three alternatively spliced isoforms of mouse Vsig1 during pre- and postnatal development of stomach and potential function of Vsig1 in differentiation of gastric epithelia. VSIG1 is required for the establishment of glandular versus squamous epithelia in the stomach.

Human GPA34/VSIG1 Protein (GPA-HM234) Specifications/Details

Protein	GPA34/VSIG1
Synonyms	Glycoprotein A34; Gpa34; dJ889N15.1; MGC44287,1700062D20Rik; VSIG1
Accession	Q86XK7-1
Species	Human
Biotinylated	no
Amino Acid Range	Val22-Glu232
Molecular Weight	The protein has a predicted MW of 48.86 kDa. Due to glycosylation, the protein migrates to 65-85 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human GPA34, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPA34 Antibody, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA.
Product Tag	C-hFc (IgG1)
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human GPA34, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPA34 Antibody, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA.
	Human GPA34 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human GPA34 is greater than 95% as determined by SEC-HPLC.