Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Tetramer Protein (MHC-HM409T)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 93% as determined by HPLC
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and FLLTRILTI peptide

Catalog: MHC-HM409T

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Product Description Recombinant Human HBV(HLA-A*02:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and FLLTRILTI peptide.

Product Background

Hepatitis B virus (HBV), is the leading cause of liver diseases infecting an estimated 240 million persons worldwide. The HBV prevalence rates are variables between different countries, with an high level of endemicity in the south-eastern part of Europe. Seven main HBV-D subgenotypes have been described until now (D1-D7).

Product Category

Recombinant Protein

Target

HBV (HLA-A*02:01)
Synonyms Hepatitis B virus;
Accession
MHC Form Tetramer
Allele HLA-A*02:01

Species

Human

Amino Acid Range

Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and FLLTRILTI peptide
Molecular Weight The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 93% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The purity of Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Tetramer is greater than 93% as determined by SEC-HPLC.
Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Tetramer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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