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Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Tetramer Protein (MHC-HM409T) Summary
Product Description |
Recombinant Human HBV(HLA-A*02:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FLLTRILTI peptide. |
Product Background |
Hepatitis B virus (HBV), is the leading cause of liver diseases infecting an estimated 240 million persons worldwide. The HBV prevalence rates are variables between different countries, with an high level of endemicity in the south-eastern part of Europe. Seven main HBV-D subgenotypes have been described until now (D1-D7). |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs |
HLA-A*02:01&B2M&HBV (FLLTRILTI) Specifications
Protein |
HLA-A*02:01&B2M&HBV (FLLTRILTI) |
Synonyms |
Hepatitis B virus; MHC; HBV |
MHC Form |
Tetramer |
Allele |
HLA-A*02:01 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FLLTRILTI peptide |
Molecular Weight |
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result. |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Tetramer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Tetramer is greater than 90% as determined by SEC-HPLC. |
Additional Information
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