Human HGF Protein (HGF-HM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human HGF, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 23.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln32-Ser728

Catalog: HGF-HM101

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Product Description Recombinant Human HGF Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln32-Ser728.

Product Background

Hepatocyte growth factor (HGF) is an important component of the pathophysiology of IR, with increased levels in most common IR conditions, including obesity. HGF has a role in the metabolic flux of glucose in different insulin sensitive cell types.

Product Category

Recombinant Protein

Target

HGF
Synonyms Hepatopoietin-A; SF; HPTA; HGF; DFNB39; F-TCF; F-TCFB; HGFB

Gene/Protein ID

P14210-1

Species

Human

Exact Sequence

Gln32-Ser728
Molecular Weight The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain.The protein has a predicted MW of 79.6 kDa (after cleavage of the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa and 30-40 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result.

Activity

Immobilized Human HGF, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 23.7ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human HGF, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 23.7ng/ml determined by ELISA.
The purity of Human HGF Protein is greater than 95% as determined by SEC-HPLC.
Human HGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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