Product Description |
Recombinant Human HGF Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln32-Ser728. |
Product Background |
Hepatocyte growth factor (HGF) is an important component of the pathophysiology of IR, with increased levels in most common IR conditions, including obesity. HGF has a role in the metabolic flux of glucose in different insulin sensitive cell types. |
Product Category |
Recombinant Protein |
Target |
HGF |
Synonyms |
Hepatopoietin-A; SF; HPTA; HGF; DFNB39; F-TCF; F-TCFB; HGFB |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln32-Ser728 |
Molecular Weight |
The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain.The protein has a predicted MW of 79.6 kDa (after cleavage of the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa and 30-40 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result. |
Activity |
Immobilized Human HGF, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human HGF, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. |
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Human HGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human HGF Protein is greater than 95% as determined by SEC-HPLC. |
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