Human HGF Protein (HGF-HM201)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human HGF R, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 26.3 ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln32-Ser728

Catalog: HGF-HM201

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Product Description Recombinant Human HGF Protein is expressed from HEK293 with hFc tag at the C-Terminus.; It contains Gln32-Ser728.

Product Background

Hepatocyte growth factor (HGF) is an important component of the pathophysiology of IR, with increased levels in most common IR conditions, including obesity. HGF has a role in the metabolic flux of glucose in different insulin sensitive cell types; plays a key role in β-cell homeostasis; and is capable of modulating the inflammatory response.HGF plays a central role in these metabolic disorders,HGF levels could be employed as a biomarker for disease status/progression, and HGF/c-Met signaling pathway modulators could effectively regulate IR and treat diabetes.

Product Category

Recombinant Protein

Target

HGF
Synonyms Hepatopoietin-A; SF; HPTA; HGF; DFNB39; F-TCF; F-TCFB; HGFB
Accession

Species

Human

Amino Acid Range

Gln32-Ser728
Molecular Weight The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain. The protein has a predicted MW of 79.6 kDa (after cleavage of the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result.

Activity

Immobilized Human HGF R, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 26.3 ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1 EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS, 100 mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human HGF R, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 26.3 ng/ml determined by ELISA.
The purity of Human HGF is greater than 95% as determined by SEC-HPLC.
Human HGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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