Human HGF Protein (HGF-HM201)
Catalog: HGF-HM201
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Product Description | Recombinant Human HGF Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln32-Ser728. |
Product Category | Recombinant Protein |
Target | HGF |
Synonyms | Hepatopoietin-A; SF; HPTA; HGF; DFNB39; F-TCF; F-TCFB; HGFB |
Gene/Protein ID | P14210-1 |
Species | Human |
Exact Sequence | Gln32-Ser728 |
Molecular Weight | The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain.The protein has a predicted MW of 79.6 kDa (after cleavage of the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result. |
Activity | Immobilized Human HGF R, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA. See testing image for detail. |
Product Tag | C-hFc |
Express System | HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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