Human HGF Protein (HGF-HM201)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human HGF R, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln32-Ser728

Catalog: HGF-HM201

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Product Description Recombinant Human HGF Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln32-Ser728.
Product Category Recombinant Protein
Target HGF
Synonyms Hepatopoietin-A; SF; HPTA; HGF; DFNB39; F-TCF; F-TCFB; HGFB
Gene/Protein ID P14210-1
Species Human
Exact Sequence Gln32-Ser728
Molecular Weight The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain.The protein has a predicted MW of 79.6 kDa (after cleavage of the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result.
Activity Immobilized Human HGF R, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human HGF R, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA.
The purity of Human HGF is greater than 95% as determined by SEC-HPLC.
Human HGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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