Name: Human HGF Protein, Ultra Low Endotoxin (HGF-HM201-UL)
Brand: KactusBio
SKU: HGF-HM201-UL-100UG
Price: 417.0 USD
Availability: InStock

Human HGF Protein, Ultra Low Endotoxin (HGF-HM201-UL)

Immobilized Human HGF R, His Tag at 2ug/ml (100 ul/Well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 10.0 ng/ml determined by ELISA (QC Test).

Human HGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Human HGF, hFc Tag on ProA-Biosensor, can bind Human HGF R, His-Avi Tag with an affinity constant of 9.93 nM as determined in BLI assay (Gator® Prime).

The purity of Human HGF is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human HGF R, His Tag at 2ug/ml (100 ul/Well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 10.0 ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gln32-Ser728

Catalog #HGF-HM201-UL

Size

100UG

500UG

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Product Description	Recombinant Human HGF Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Gln32-Ser728.
Product Background	Hepatocyte growth factor (HGF) is an important component of the pathophysiology of IR, with increased levels in most common IR conditions, including obesity. HGF has a role in the metabolic flux of glucose in different insulin sensitive cell types; plays a key role in β-cell homeostasis; and is capable of modulating the inflammatory response.HGF plays a central role in these metabolic disorders,HGF levels could be employed as a biomarker for disease status/progression, and HGF/c-Met signaling pathway modulators could effectively regulate IR and treat diabetes.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	HGF
Synonyms	Hepatopoietin-A; SF; HPTA; HGF; DFNB39; F-TCF; F-TCFB; HGFB
Accession	P14210-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln32-Ser728
Molecular Weight	The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain. The protein has a predicted MW of 79.6 kDa (after cleavage of the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result.
Activity	Immobilized Human HGF R, His Tag at 2ug/ml (100 ul/Well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 10.0 ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS, 100 mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human HGF R, His Tag at 2ug/ml (100 ul/Well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 10.0 ng/ml determined by ELISA (QC Test).
	Human HGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human HGF, hFc Tag on ProA-Biosensor, can bind Human HGF R, His-Avi Tag with an affinity constant of 9.93 nM as determined in BLI assay (Gator® Prime).
	The purity of Human HGF is greater than 95% as determined by SEC-HPLC.