Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer Protein (MHC-HE017)

Immobilized Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.3ng/ml determined by ELISA.

Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer is greater than 95% as determined by SEC-HPLC.

Express System: E.coli
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.3ng/ml determined by ELISA.
Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FMNKFIYEI peptide

Catalog #MHC-HE017

Price

$341⁰⁰

Size

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Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer Protein (MHC-HE017) Summary

Product Description

Recombinant Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FMNKFIYEI peptide.

Product Background

Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response.

HLA-A*02:01&B2M&AFP (FMNKFIYEI) Specifications

Protein	HLA-A*02:01&B2M&AFP (FMNKFIYEI)
Synonyms	Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-feto; AFP; HPAFP; AFPD; FETA;
MHC Form	Monomer
Allele	HLA-A*02:01
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&FMNKFIYEI
Species	Human
Biotinylated	no
Amino Acid Range	Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FMNKFIYEI peptide
Molecular Weight	The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Activity	Immobilized Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.3ng/ml determined by ELISA.
Product Tag	C-His-Avi
Fluorophore	Unconjugated
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.3ng/ml determined by ELISA.
	Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer is greater than 95% as determined by SEC-HPLC.