• Express System: E.coli
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FLLPTGAEA peptide
Catalog #MHC-HE452
Price
Regular price $34100
Regular price Sale price $34100
/
Size
Avantor VWR Fisher Scientific

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Human HLA-A*02:01&B2M&CG1 (FLLPTGAEA) Monomer Protein (MHC-HE452) Summary

Product Description

 Recombinant Human HLA-A*02:01&B2M&CG1 (FLLPTGAEA) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FLLPTGAEA peptide.

Product Background

 Cathepsin G (CG) is a myeloid azurophil granule protease that is highly expressed by acute myeloid leukemia (AML) blasts and leukemia stem cells. CG1 (FLLPTGAEA) is a human leukocyte antigen-A2-restricted nonameric peptide derived from CG that can act as an immunogenic target in AML.

HLA-A*02:01&B2M&CG1 (FLLPTGAEA) Specifications

Protein
 HLA-A*02:01&B2M&CG1 (FLLPTGAEA)
Synonyms
 CG1 (FLLPTGAEA)
MHC Form
 Monomer
Allele
 HLA-A*02:01
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FLLPTGAEA peptide
Molecular Weight
 The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Product Tag
 C-His-Avi
Fluorophore
 Unconjugated
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human HLA-A*02:01&B2M&CG1 (FLLPTGAEA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human HLA-A*02:01&B2M&CG1 (FLLPTGAEA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&CG1 (FLLPTGAEA) Monomer is greater than 95% as determined by SEC-HPLC. The purity of Human HLA-A*02:01&B2M&CG1 (FLLPTGAEA) Monomer is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...