Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein (MHC-HM458)
Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein (MHC-HM458)
Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein (MHC-HM458)
Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein (MHC-HM458)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and NLVPMVATV peptide
Catalog #MHC-HM458
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Product Description
 Recombinant Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and NLVPMVATV peptide.
Product Background
 Human cytomegalovirus (CMV) is one of the major causes of severe complications in immunocompromised patients, such as recipients of hematopoietic cells, solid organ transplantation, and HIV-infected individuals. CMV is also a major pathogen in congenital infection. It can cause life-threatening diseases and severe neurological sequelae in newborn infants. CMV phosphoprotein pp65 is thought to be a major antigen for CMV-specific cellular immunity.
Product Category
 Recombinant Protein
Target
 HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer
MHC Form
 Monomer
Allele
 HLA-A*02:01
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and NLVPMVATV peptide
Molecular Weight
 The protein has a predicted MW of 50.35 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer is greater than 95% as determined by SEC-HPLC.
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