Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Tetramer Protein (MHC-HE013

Express System: E.coli
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and NLVPMVATV peptide

Catalog #MHC-HE013T

Price

$825⁰⁰

Size

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Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Tetramer Protein (MHC-HE013T) Summary

Product Description

Recombinant Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Tetramer Protein is expressed from E.coli with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and NLVPMVATV peptide.

Product Background

Human cytomegalovirus (CMV), a β-herpes virus with a double-stranded DNA, infects a wide variety of cells and establishes latency in the host. CMVpp65, a tegument protein of the herpes virus CMV, is the main viral antigen found in peripheral blood mononuclear cells (PBMCs) after viral infection and may activate cell-mediated immunity, accounting for 70-90% of the cytotoxic CD8+ T cells' (CTLs) response to CMV. Among the pp65-derived CTL epitope peptides, the 9-mer peptide 495NLVPMVATV503 (CMVpp65 495-503 peptide) is the most immunogenic T cell epitope predominantly displayed on HLA-A*02:01, the most common MHC-I allele in the population.

HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Specifications

Protein	HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV)
Synonyms	CMVpp65; NLVPMVATV
MHC Form	Tetramer
Allele	HLA-A*02:01
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&NLVPMVATV
Species	Human
Biotinylated	no
Amino Acid Range	Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and NLVPMVATV peptide
Molecular Weight	The protein has a predicted MW of 258 kDa. The protein migrates to 120-180 kDa (HLA-A*02:01&streptavidin tetramer) and 11.9 kDa (B2M) based on Bis-Tris PAGE result under Non reduced (N) condition.
Product Tag	C-His-Avi
Fluorophore	Unconjugated
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.
	The purity of Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Tetramer is greater than 95% as determined by SEC-HPLC.