Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Tetramer Protein (MHC-HE013T)
Catalog: MHC-HE013T
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| Product Description | Recombinant Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Tetramer Protein is expressed from E.coli with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and NLVPMVATV peptide. |
Product Background |
Human cytomegalovirus (CMV), a β-herpes virus with a double-stranded DNA, infects a wide variety of cells and establishes latency in the host. CMVpp65, a tegument protein of the herpes virus CMV, is the main viral antigen found in peripheral blood mononuclear cells (PBMCs) after viral infection and may activate cell-mediated immunity, accounting for 70-90% of the cytotoxic CD8+ T cells' (CTLs) response to CMV. Among the pp65-derived CTL epitope peptides, the 9-mer peptide 495NLVPMVATV503 (CMVpp65 495-503 peptide) is the most immunogenic T cell epitope predominantly displayed on HLA-A*02:01, the most common MHC-I allele in the population. |
Product Category |
Recombinant Protein |
Target |
HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Tetramer |
| MHC Form | Tetramer |
| Allele | HLA-A*02:01 |
Gene/Protein ID |
A0A140T913(HLA-A*02:01)&P61769(B2M)&NLVPMVATV |
Species |
Human |
Exact Sequence |
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and NLVPMVATV peptide |
| Molecular Weight | The protein has a predicted MW of 258 kDa. The protein migrates to 120-180 kDa (HLA-A*02:01&streptavidin tetramer) and 11.9 kDa (B2M) based on Tris-Bis PAGE result under Non reducing (N) condition. |
Product Tag |
C-His-Avi |
Express System |
E.coli |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Liquid |
| Shipping | Shipped with dry ice. |
| Formulation | Supplied as 0.22 um filtered solution in PBS (pH 7.4). |
| Stability And Storage | Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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The purity of Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Tetramer is greater than 95% as determined by SEC-HPLC. |
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Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%. |
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