• Express System: E.coli
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&MAGE-A4 Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA.
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and GVYDGREHTV peptide
Catalog #MHC-HE047
Price
Regular price $34100
Regular price Sale price $34100
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Size
Avantor VWR Fisher Scientific

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Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer Protein (MHC-HE047) Summary

Product Description

 Recombinant Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and GVYDGREHTV peptide.

Product Background

 Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene.The MAGE- A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A*0201 MAGE-A4 (GVYDGREHTV) complex Protein is a complex of HLA-A*0201 of -the MHC Class I, B2M and GVYDGREHTV peptide of -the MAGE-A4.

HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Specifications

Protein
 HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV)
Synonyms
 HLA0201; MHC I; MAGE-A4; CT1.4; MAGE4A; MAGE4B; MAGE-X2; member 4
MHC Form
 Monomer
Allele
 HLA-A*02:01
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and GVYDGREHTV peptide
Molecular Weight
 The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Activity
 Immobilized Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&MAGE-A4 Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Unconjugated
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&MAGE-A4 Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA. Immobilized Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&MAGE-A4 Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA.
Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Anti-HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Antibody, hFc Tag on ProA-Biosensor can bind Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer, His Tag with an affinity constant of 16.40 nM as determined in BLI assay . Loaded Anti-HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Antibody, hFc Tag on ProA-Biosensor can bind Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer, His Tag with an affinity constant of 16.40 nM as determined in BLI assay .
The purity of Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer is greater than 95% as determined by SEC-HPLC. The purity of Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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