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Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer Protein (MHC-HE047) Summary
Product Description |
Recombinant Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and GVYDGREHTV peptide. |
Product Background |
Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene.The MAGE- A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A*0201 MAGE-A4 (GVYDGREHTV) complex Protein is a complex of HLA-A*0201 of -the MHC Class I, B2M and GVYDGREHTV peptide of -the MAGE-A4. |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs |
HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Specifications
Protein |
HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) |
Synonyms |
HLA0201; MHC I; MAGE-A4; CT1.4; MAGE4A; MAGE4B; MAGE-X2; member 4 |
MHC Form |
Monomer |
Allele |
HLA-A*02:01 |
Accession |
A0A140T913(HLA-A*02:01)&P61769(B2M)&GVYDGREHTV |
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and GVYDGREHTV peptide |
Molecular Weight |
The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result. |
Activity |
Immobilized Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&MAGE-A4 Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&MAGE-A4 Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA. |
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Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Anti-HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Antibody, hFc Tag on ProA-Biosensor can bind Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer, His Tag with an affinity constant of 16.40 nM as determined in BLI assay . |
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The purity of Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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